GPC/SEC Chromatography

Gel Permeation Chromatography (GPC), also Size Exclusion Chromatography (SEC) separates particles according to their hydrodynamic volume (hydrodynamic radius).

Unlike HPLC where sorting occurs based on chemical or physical interaction, separation in GPC/SEC should occur solely on steric properties.

Based on these different mechanics of separation it is obvious that optimum results in size determination will be reached through use of optimized instrumentation for GPC – not only by the choice of column.

Dedicated GPC/SEC systems among other differences offer:

  • lower column back pressure
  • better pulsation control including compressibility correction
  • different selection of detectors

One key advantage of modern GPC/SEC is the possibility to determine absolute size without the requirement for calibration of the system using angle dependent light scattering information.